Phylogenetic tree reconstructed using the partial coat protein gene fragment amplified using polyvalent RT-PCR1 assay.

<p>The name of the isolate is followed by information on the host of origin, the sampling site and the year of collection. Hosts are given as follows: PC, <i>Poa cookii</i>, PA, <i>Poa annua</i>, FC, <i>Festuca contracta</i>, AM, <i>Agrostis magellanica</i>. Corresponding sequences from representative <i>Luteoviridae</i> members retrieved from GenBank were included in the analysis and the relevant accession numbers are indicated. The abbreviations used are: PAV: BYDV-PAV; MAV: BYDV-MAV; SGV: BYDV-SGV; RMV: BYDV-RMV; GPV: BYDV-GPV; RSDaV: <i>Rose spring dwarf associated virus</i>; BChV: <i>Beet chlorosis virus</i>; SbDV: <i>Soybean dwarf virus</i>; BLRV: <i>Bean leaf roll virus</i>; PLRV: <i>Potato leaf roll virus</i>; PEMV: <i>Pea enation mosaic virus</i>. The tree was reconstructed by using the neighbor-joining method, using the p-distance model and was bootstrapped with 1,000 replications. Numbers at nodes indicate bootstrap values higher than 70%. The scale bar represents 5% nucleotide divergence.