Experimental design for competition experiments.

<p>Mixtures of <i>Vibrio fischeri</i> wild type (MJ1) and <i>luxI</i> mutant (MJ211) strains at optical density based starting ratios of 1∶1 or 1∶10 were inoculated (100 µl) into 5 ml of fresh media in triplicate. Each replicate culture was subsequently subcultured daily for ten days and wild type to mutant ratios determined every second day.