Lqf is required for synaptic overgrowth and pMad retrograde transport in <i>spinster</i> mutants.

<p>(A–F) Representative images of bouton morphology and pMad levels at the NMJ (A, C, E) and motoneuron nuclei (B, D, F) in <i>Drosophila</i> 3^rd instar larval NMJs from control larvae (CS, A–B), <i>spin</i> mutants (C–D) and <i>spin;lqf</i> double mutants (E–F). Synaptic boutons are overgrown in <i>spin</i> mutants (C), and this overgrowth is suppressed in the <i>spin;lqf</i> double mutants (E). (G) Quantification of synaptic bouton number in <i>spin</i> and <i>spin;lqf</i> mutants. Taken from three different animals for each genotype, n values represent the number of NMJs quantified. (H) Quantification of pMad intensity in boutons (white bars) and motoneuron nuclei (black bars). n = 5 NMJs from three larvae and 20 nuclei from five different larvae. Error bars represent SEM. *P<0.05, **P<0.01, ***P<0.001. One-way ANOVA with Tukey's Multiple Comparison Post test. Control (CS), <i>spin</i> (<i>spin⁴/spin⁵</i>), <i>spin, lqf</i> (<i>spin⁴/spin⁵; lqf^ARI/lqf Df</i>).