MANS peptide treatment attenuates migration of NIH-3T3 fibroblasts.

<p>NIH-3T3 fibroblasts were grown to confluency on fibronectin (A&B) or collagen (C) coated coverslips and scratch assays were performed with increasing concentrations (1, 50 or 100 µM) of MANS or RNS, VC (PBS) or 100 nM wortmannin. (A) Photos are representative of experiments on fibronectin-coated coverslips; bar is equivalent to 500 µm in length. The average percent wound closure is shown on fibronectin (B) or collagen substrates (C), with four individual experiments for both substrates performed. Statistical analysis (p<0.05) was performed where “a” denotes a significantly decreased ability to migrate back into the wound relative to no treatment and “b”, “c”, and “d” denote a statistically reduced ability to migrate back into the wound relative to 1 µM, 50 µM and 100 µM RNS, respectively.