<p>(A) Schematic representation of three mouse <i>Pparγ</i> cDNAs. The positions of unique forward primers for each transcript and common reverse primer binding sites for qPCR analyses are indicated as arrows. The size of each PCR product is indicated in parenthesis. (B) Expression levels of <i>Pparγ1sv</i>, <i>Pparγ1</i>, and <i>Pparγ2</i> transcripts in mouse tissues were evaluated by qPCR analysis using the normalized first strand cDNAs as a template. The values represent the mean with error bars of triplicate measurements. (C) Expression levels of <i>Pparγ1sv</i>, <i>Pparγ1</i>, and <i>Pparγ2</i> transcripts in mouse white adipose tissue and brown adipose tissue. The values are normalized to the amount of 18S ribosomal RNA. The column d...
<p>(A) Schematic illustration of primer design for detection of the foxed PPARγ allele (primers S1 a...
<p>Mice were sacrificed on day 28 and femurs collected for RNA purification as described in methods....
<p>(A-B) PPARα is a major PPAR isoform in the heart. Relative copy numbers of PPAR isoforms at the m...
<p>Semi- quantitative RT-PCR (<b>A</b>) and Western blot (<b>B</b>) analysis of PPARγ expression in ...
<p>(<b>A</b>) Comparative analysis of 100 Kb of sequence upstream of the PPARγ2 transcriptional star...
<p><b><i>A. Murine Tissues.</i></b> qPCR analysis of Dies1 transcript level in a panel of 12 murine ...
<p>(A–D) <i>Leptin, Zfp423, PPAR</i> and <i>aP2</i> gene expression was quantified by qPCR in each g...
<p>Real-time qPCR analysis of <i>LacZ</i> (<b>A</b>), PPARγ (<b>B</b>), and adiponectin (<b>C</b>) e...
(A) Detection of wild type (wt, 700 bp) and mutant (mt, 300 bp) Pparg transcripts by RT-PCR. Note th...
<p>A. Summary of PPARα activation or suppression. The PPARα signature was compared to ~1850 biosets ...
<p>(<b>A</b>) Tissue distribution of <i>LacZ</i> mRNA expression in PPARγ (+/−) and PPARγ CS1-5_<i>L...
<p>Mice were sacrificed on day 28 and femurs collected for RNA purification as described in methods....
<p>Column headings represent; WT = wild type mice, HYP = X-linked hypophosphatemic rickets mice,...
The reliability of reverse transcription-quantitative PCR (RT-qPCR) results in gene expression studi...
<p>Primer sequences for mouse mRNAs measured by real-time PCR (The primer sequences are listed from ...
<p>(A) Schematic illustration of primer design for detection of the foxed PPARγ allele (primers S1 a...
<p>Mice were sacrificed on day 28 and femurs collected for RNA purification as described in methods....
<p>(A-B) PPARα is a major PPAR isoform in the heart. Relative copy numbers of PPAR isoforms at the m...
<p>Semi- quantitative RT-PCR (<b>A</b>) and Western blot (<b>B</b>) analysis of PPARγ expression in ...
<p>(<b>A</b>) Comparative analysis of 100 Kb of sequence upstream of the PPARγ2 transcriptional star...
<p><b><i>A. Murine Tissues.</i></b> qPCR analysis of Dies1 transcript level in a panel of 12 murine ...
<p>(A–D) <i>Leptin, Zfp423, PPAR</i> and <i>aP2</i> gene expression was quantified by qPCR in each g...
<p>Real-time qPCR analysis of <i>LacZ</i> (<b>A</b>), PPARγ (<b>B</b>), and adiponectin (<b>C</b>) e...
(A) Detection of wild type (wt, 700 bp) and mutant (mt, 300 bp) Pparg transcripts by RT-PCR. Note th...
<p>A. Summary of PPARα activation or suppression. The PPARα signature was compared to ~1850 biosets ...
<p>(<b>A</b>) Tissue distribution of <i>LacZ</i> mRNA expression in PPARγ (+/−) and PPARγ CS1-5_<i>L...
<p>Mice were sacrificed on day 28 and femurs collected for RNA purification as described in methods....
<p>Column headings represent; WT = wild type mice, HYP = X-linked hypophosphatemic rickets mice,...
The reliability of reverse transcription-quantitative PCR (RT-qPCR) results in gene expression studi...
<p>Primer sequences for mouse mRNAs measured by real-time PCR (The primer sequences are listed from ...
<p>(A) Schematic illustration of primer design for detection of the foxed PPARγ allele (primers S1 a...
<p>Mice were sacrificed on day 28 and femurs collected for RNA purification as described in methods....
<p>(A-B) PPARα is a major PPAR isoform in the heart. Relative copy numbers of PPAR isoforms at the m...