ND-GFP-expressing cells proliferated in the sciatic nerve and formed fibers in 3D Gelfoam® histoculture.

<p>(A) Time-course imaging of ND-GFP-expressing cell trafficking in the histocultured sciatic nerve from an ND-GFP mouse. The nerve became enriched with ND-GFP-expressing cells by day 14. At day 6, the ND-GFP-expressing cells proliferated in extending fibers and migrated into the Gelfoam®. At day 14, many fibers consisting of ND-GFP-expressing cells extended widely and radially around the nerve. Bar: 500 µm. (B) Magnified images of the growing nerve. At day 6, the ND-GFP-expressing cells proliferated as fibers and migrated into the Gelfoam®. Bar: 100 µm. (C) High-magnification images of the area inside the boxes in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067153#pone-0067153-g003" target="_blank">Figure 3B</a>. At day 9, the ND-GFP-expressing cells grew in fibers (white arrow heads). Some spherical cells proliferated in the growing fibers. They did not express nestin (white arrows). At day 14, the spindle cells expressed ND-GFP (white arrow head). In addition, there were spherical cells without nestin expression (white arrow). Bar: 10 µm.