ND-GFP-expressing cells are located in dorsal peripheral nerve roots but not in the spinal cord in 3D Gelfoam® histoculture.

<p>(A) A spinal cord with posterior roots removed from an ND-GFP transgenic mouse was put in Gelfoam® histoculture. On day 0, some ND-GFP-expressing cells were observed in the origin of posterior root (enclosed by white dashed lines). (B) Cross section of the spinal cord just after removal show that there were a few ND-GFP-expressing cells in the posterior root. White bar: 50 µm. (C) At day 7, posterior roots were enriched with ND-GFP-expressing cells. (D) Cross section of the spinal cord cultured for 7 days show that ND-GFP-expressing cells proliferated in the posterior root but not in the cortex of spinal cord. White bar: 50 µm.