Identification of parvalbumin-positive interneurons and effect of BDNF on firing properties.

<p>A. Confocal image showing the co-localization of an intracellularly biocytin-labeled interneuron (green) and immunoreactivity for parvalbumin (red). B. Current-clamp recording from a parvalbumin-positive interneuron showing the firing before and after 10 min of bath perfusion of BDNF (15 ng/ml). The neuron was depolarized by a 200 pA current injection for 500 ms. Arrowhead indicates a delayed firing observed in the interneuron near the current threshold. C. The time course of the evoked firing (black y axis) and resting membrane potential (blue y axis) of an interneuron exposed to BDNF (black and blue circles) and a time-matched control (black and blue squares) exposed to control ACSF. The time of BDNF application is indicated by the solid line. D. Relationship between the firing frequency versus increasing current steps (0–400 pA) for control (squares) and after 10 min of BDNF exposure (circles) (n = 8).