Monomerization of eNOS and nNOS is augmented in ECM.

<p><b>A–C</b>: Non-denatured and non-boiled brain lysates from ECM and uninfected mice were probed for respective NOS isoforms. Monomer to dimer ratios are shown by bar graphs. Representative blots for each condition are shown above. Ratios of ECM brains were significantly increased from those of uninfected brains for eNOS and nNOS but not iNOS. <b>D–F</b>: Bar graphs represent ratio of amount of each NOS monomeric form to total amount of β-tubulin. Though not statistically significant (<i>P</i> = 0.059), amount of eNOS monomers increased by approximately two folds in ECM. Significantly elevated amount of nNOS but not iNOS monomers were also observed during ECM development. A total of 9–10 uninfected and 8–9 ECM mouse brains were analyzed for each NOS isoform. *<i>P</i><0.05, NS: non significant.