Lack of endogenous IL-10 results in higher NF-κB activation and production of pro-inflammatory cytokines by macrophages infected with <i>B. abortus</i>.

<p>(<b>A</b>) NF-κB activation measured in RAW-Blue macrophages infected with <i>B. abortus</i> 2308 for 8 h and 24 h in the presence of IL-10 receptor blocking antibody (IL-10R Ab), isotype control (IgG Ab) or exogenous IL-10 (rIL-10). (<b>B</b>) qRT-PCR analysis of IL-6, and TNF-α expression by BMDM from wild type mice (C57BL/6) and IL-10 deficient mice (IL-10^−/−) in the presence or absence of recombinant IL-10. (<b>C</b>) ELISA essay for IL-6 and TNF-α production in supernatant from wild type mice (C57BL/6) and IL-10 deficient mice (IL-10^−/−) in the presence or absence of recombinant IL-10. Results shown are representative of two independent experiments. n = 5. Values represent mean ± SEM. *P<0.05 using unpaired t-test statistical analysis.