Major neuronal miRNA functions in repressing dauer formation are independent of serotonin activity.

<p><b>A–C.</b> Bar graphs representing the percent of dauer progeny of indicated genotypes. <b>A.</b> All mutants were assayed in the same experiments. <b>B. </b><i>unc-3(e151 lf) ain-1(ku322 lf)</i> and <i>unc-3(e151 lf) ain-1(ku322 lf) lim-4(yz12 lf)</i> were scored in separate assays from the <i>unc-3(lf) lim-4(lf)</i> mutant. <b>C.</b> Original <i>tph-1(mg280 lf)</i> mutants were used in this experiment (not siblings of <i>ain-1(lf); tph-1(lf)</i> mutants). Error bars represent S.E.M. from at least three biological replicates. A student's t-test was used to determine statistical significance for all experiments. <b>D.</b> Brief pathway outlining the relationship of serotonin, cGMP, IIS, and TGF-β in relation to dauer formation.