Binding pattern of CLL69 rAbs to OSE.

<p>(<b>A</b>) Chemiluminescent ELISA of CLL69 rAbs (1 µg/mL) binding to plated BSA, MAA- and MDA-modified BSA, MAA- or MDA-modified LDL, copper oxidized LDL (OxLDL), phosphocholine-modified BSA (PC-BSA), or native LDL (LDL), each at 5 µg/mL, for 1.5 hour at RT. Binding was determined as in methods, expressed as relative light units (RLU) per 100 milliseconds (ms) and represent at least three independent experiments in triplicate determinations (mean ± SD). (<b>B</b>) Competition immunoassay for the specificity of CLL69C rAb binding to MAA- epitopes. A fixed and limiting dilution of CLL69C rAb was incubated in the absence and presence of increasing amounts of indicated competitors and the extent of binding to plated MAA-LDL (left panel), MAA-BSA (middle panel) or MDA-LDL (right panel) was determined. Data are expressed as a ratio of binding in the presence of competitor (B) divided by absence of competitor (B₀). Data are representative of three independent experiments, each determined in triplicate.