The involvement of CRT-STAT3 pathway in FZD induced mitochondria and cardiomyocytes injury.

<p>When neonatal rat cardiomyocytes (NRCMs) were exposed to FZD for 48 h, dose-dependent decreases in cell viability (A) and MMP (B) were observed at the indicated concentration. C: After NRCMs were incubated with FZD for 48 h, annexin V-FITC based apoptosis assay revealed that apoptosis rate was increased in a dose-dependent manner. D: When NRCMs were incubated with FZD for 24 h, real time PCR revealed that FZD regulated the mRNA expression of CRT and MnSOD on a dose-dependent manner, but had no significant effect on the mRNA expression of STAT3. E: After 24 h stimulation with FZD, the protein levels of CRT, phosphorylated and total STAT3, and MnSOD in the whole cell lysates were determined by western blot. β-actin was used as a loading control. A representative blot is shown for each condition. F: Western blot was used to determine the protein expression of phosphorylated and total STAT3 in cardiac mitochondria isolated from NRCMs exposed to FZD for 24 h. COXIV was used as a loading control for the mitochondrial protein. A representative blot is shown for each condition. All statistical data were obtained from 3–5 independent experiments, and represented as the mean ± SD. *<i>P</i><0.05 versus the control group.