FcγR deficiency inhibits foam cell formation and pro-inflammatory gene expression in peritoneal macrophages.

<p>(<b>A</b>) Macrophages from ApoE^−/− and γ^−/−ApoE^−/− mice were exposed to oxLDL alone (25 µg/mL) and oxLDL-IC (IgG1 and IgG2, 1∶0.5 ratio) for 6 h. Cells containing oil-red-O stained fat droplets were considered foam cells. Representative images (original magnification x400) and macrophage foam cell quantification are shown. (<b>B</b>) Real-time PCR analysis of scavenger receptors (CD36 and SR-A) in freshly isolated peritoneal macrophages. Values corrected by 18S expression are expressed as arbitrary units (a.u.). (<b>C</b>) Expression levels of M1 and M2 response genes in macrophages from WT, ApoE^−/− and γ^−/−ApoE^−/− mice. Data are normalized to WT values ( = 1; dashed line). (<b>D</b>) Western blot analysis of Arg isoforms in peritoneal macrophages from WT, ApoE^−/− and γ^−/−ApoE^−/− mice. Data of densitometry are corrected by loading control (β-actin) and expressed as Arg2/Arg1 ratio. Bars represent the mean±SEM of n = 5–7 animals per group. *<i>P</i><0.05 versus Basal; # <i>P</i><0.05 versus ApoE^−/− cells.