Effect of FcγR deficiency on macrophage phenotype in atherosclerotic lesions.

<p>(<b>A</b>) Immunofluorescence analysis of total macrophages (Mac-3; red), and phenotypes M1 and M2 (Arg2 and Arg1, respectively; green) in aortic sections from transplanted mice. Representative photomicrographs (top panel, magnification x200) and high-magnification images (x400) of the selected regions (squares). (<b>B</b>) Quantification of Arg2⁺ and Arg1⁺ macrophages per lesion area. Values represent the mean±SEM of total number of animals per group. (<b>C</b>) Real-time PCR analysis of Arg isoforms in aortic tissue. Correlation between Arg2/Arg1 expression and lesion area in A→A (filled circles, n = 16) and γA→A (open circles, n = 17) mice is shown.