Histological analysis, lipid and TNF-α content in liver of young rats on control (CD) and high fat (HFD) diet for 5 or 8 weeks.

<p>Liver sections were stained with (<b>A</b>) hematoxylin-eosin or (<b>B</b>) Oil red O. Micrographs in both panels are representative pictures with magnification 400 X. (<b>C</b>) Quantitative analysis of liver TGL content obtained from all animals is expressed as mg/dl in 500 mg tissue. (<b>D</b>) Real time-PCR of TNF-α mRNA is shown. **P<0.01 and ***P<0.001 vs CD; #P<0.05 vs HFD 5 weeks.