Impaired intestinal barrier and ER stress in<i>Mttp-IKO</i> mice.

<p><b>A</b>. <i>Mttp-IKO</i> and control mice either before DSS or 7 days after DSS treatment were orally administered FITC-dextran and serum levels measured 4 hrs later. Data are the mean ± SEM of 7–8 mice per group.*P<0.05. <b>B. and C.</b> mRNA expression of genes related to ER stress and epithelial barrier functioin in small intestine and colon. mRNA expression was measured by QPCR and expressed as fold change compared to non-DSS treated control mice after normalized to internal control Gapdh. Data are presented as the mean ± SEM of 4–5 mice per group. <b>D</b>. The effect of TUDCA on survival proportion of Control and <i>Mttp-IKO</i> mice on DSS. 8–10 week old mice were treated with TUDCA or saline intraperitoneally during simultaneous administration of DSS (2.5%) in drinking water for 12 days. n = 5–9 each group. *p<0.05.