GA CD8⁺ T cells are necessary to induce CD4⁺ Tregs <i>in vivo</i><b>.</b>

<p> (A) GA- or PBS-treated (control) bone marrow macrophages were adoptively transferred into wild-type or CD8^−/− recipient mice at peak disease. Twenty five days post-EAE induction, splenic CD4⁺CD25⁺Foxp3⁺ regulatory T cells were quantified by flow cytometry. (B) CD8⁺ T cells derived from GA/IFA- or OVAp/IFA-immunized mice were transferred into wild-type hosts one day prior to EAE induction. After 25 days splenic CD4⁺CD25⁺Foxp3⁺ regulatory T cells were stained and quantified by flow cytometry.