RNAi of <i>CYC9</i> reveals it is essential for viability in procyclic and bloodstream form trypanosomes.

<p>A. Cumulative growth curves for two independent procyclic <i>CYC9</i> RNAi clones (clone 1: C1; clone 2: C2) cultured in the presence or absence of tetracycline (tet). B. Real time PCR analysis of <i>CYC9</i> transcript for procyclic <i>CYC9</i> RNAi clones 1 and 2 at 48 hours post-induction. Error bars represent standard deviations of three replicates. The percentage of mRNA transcript remaining is indicated. C. Cumulative growth curves for two independent bloodstream <i>CYC9</i> RNAi clones (clone 1: C1; clone 2: C2) cultured in the presence or absence of tetracycline (tet). D. Real time PCR analysis of <i>CYC9</i> transcript for bloodstream form <i>CYC9</i> RNAi clones 1 and 2 at 20 hours post-induction. Error bars represent standard deviations of three replicates.