PS2V is up-regulated under cholesterol-fed conditions.

<p>Quantitative PCR analysis shows that, in comparison to animals fed a control diet, guinea pigs fed a cholesterol rich diet showed a significant increase in <i>PSEN2</i> and <i>PS2V</i> transcripts in (<b>A</b>) frontal cortex (<i>p</i> = 0.004; t = 3.429, d.f. = 14 and <i>p</i><0.0001, t = 6.841, d.f. = 14, respectively) and (<b>B</b>) cerebellum (<i>p</i><0.005; <i>t</i> = 4.484 and <i>d.f.</i> = 14 and <i>p</i><0.003, t = 4.763, d.f. = 14, respectively). The fold increase of PS2V levels in these regions was greater than the increase in full length <i>PSEN2</i> levels [4 fold vs 2 fold in frontal cortex (<i>p</i> = 0.01, <i>t</i> = 2.994, <i>d.f.</i> = 14], and 6 fold vs 2 fold in the cerebellum (<i>p</i> = 0.002, <i>t</i> = 3.733, <i>d.f.</i> = 14)]. Data is represented as fold change from control fed animals. Transcript levels were normalised against RPS16. Data represents ± SEM.