Total <i>MAPT</i> and <i>MAPT</i>3R transcripts are up-regulated under cholesterol fed conditions.

<p>Quantitative PCR analysis shows that, in comparison to animals fed a control diet, guinea pigs fed a cholesterol rich diet showed a significant increase in (A) total <i>MAPT</i> (<i>p</i> = 0.031, <i>t</i> = 3.560, <i>d.f.</i> = 14) and (B) <i>MAPT3</i>R (<i>p</i><0.0001, <i>t</i> = 6.468, <i>d.f.</i> = 14) transcripts but (C) no change was observed in <i>MAPT4R</i> transcripts (<i>p</i> = 0.1320, <i>t</i> = 1.60, <i>d.f.</i> = 14, ns). An increased 3R/4R ratio was observed (<i>p</i> = 0.0007, <i>t</i> = 4.326, <i>d.f.</i> = 14). Data is represented as fold change from control fed animals. Transcript levels were normalised against RPS16. Data represents ± SEM.