Analysis of CAV2-miR-29a interaction in humans and pigs by means of reporter gene assays.

<p>The interaction between miR-29a and human as well as porcine CAV2 was verified by means of reporter gene assays using HeLa and IPEC-J2 cells, respectively. Identified target sites between miR-29a and human (panel A) as well as porcine CAV2 (panel B) were analysed using RNAhybrid. Relative luciferase activity (Luc <i>_Gaussia</i>: Luc <i>_Cypridina</i>) was determined respective to nonsense miRNA mimics as well as mutagenised seeds (red letters) serving as controls. The columns show means of normalised luciferase activity each measured in triplicates while error bars show the standard deviation. Asterisks indicate statistical significance between samples (*: P<0.05; **: P<0.01; ***: P<0.001, paired t test).