Comparing edge detection techniques.

<p>Comparing three edge detection techniques for barrier assays with two different cell densities: 10,000 cells (A,C) and 30,000 (B,D) cells. (A–B): Comparison of the three edge detection techniques showing the mean area enclosed by the leading detected edge at , , and hours with time points indicated. Red lines correspond to the the manual edge technique using MATLAB's Image Processing Toolbox for a range of the threshold parameter . Black dots correspond to the automatic MATLAB results and the green asterisks correspond to the automatic ImageJ results. (C–D): The migration rate of cells in the barrier assays expressed as % using <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067389#pone.0067389.e004" target="_blank">equation (1</a>). Results correspond to the minimum (red) and maximum (blue) average areas detected using the manual MATLAB technique. Error bars correspond to one standard deviation about the mean.