Jurkat and Molt4 cells express high expression of 11β-HSD2 and fail to auto-induce GRα after cortisol treatment.

<p>(A) 11β-HSD2 mRNA expression of each sample was detected using RT-PCR. These samples include (from left to right): Placenta, a 11β-HSD2 positive control; Jurkat, a T-ALL cell line; Molt4, a T-ALL cell line; PBT, peripheral blood T cells from healthy volunteers; Daudi, a B-NHL cell line; Raji, a B-NHL cell line. (B) Representative western blots showed 11β-HSD2 protein expression in each sample. 50 µg protein extracts from each sample were probed with 11β-HSD2 antibody. The expression levels of β-actin in the lysates served as the loading control. An immunoreactive protein of ∼42 kDa was found in Jurkat and Molt4 cells, but not in PBT, Daudi and Raji cells. (C) Jurkat, Molt4, Daudi and Raji cells were treated with 1 µM cortisol for 24 hours. GRα levels of cortisol-treated cells relative to those of untreated cells (control) in each sample were analyzed by quantitative real-time PCR. Data are shown as mean±SD of triplicate samples. *p<0.05 <i>vs.</i> untreated cells. There was no induction of GRα after cortisol treatment in Jurkat and Molt4 cells in comparison with Daudi and Raji cells.