Nuclear dynamics of mRNA export proteins were measured by FRAP.

<p>(A) Representative images illustrating fluorescence recovery of Chtop-GFP after photobleaching in nuclear speckle (red circle) of live HeLa cells. (B) The plots of time-dependent normalized fluorescence recovery of Chtop-GFP in the bleached speckles and nucleoplasms. (C) Representative images illustrating fluorescence recovery of Alyref-GFP after photobleaching in the nuclear speckle (red circle) of live HeLa cells. (D) The plots of time-dependent normalized fluorescence recovery of Alyref-GFP in the nuclear speckle and nucleoplasm. RFU, relative fluorescent units; FRAP, fluorescence recovery after photobleaching; Scale bars, 5 µm.