Combinatorial detection of known neural intracellular markers plus a panel of candidate surface antigens in human cell lines.

<p>(<b>A</b>) In SH-SY5Y cells CD49f, CD90^HIGH and CD29 expression clustered distinct from doublecortin (DCX)-positivity, while subsets of CD56, CD24 and CD200 closely correlated with neuronal differentiation (DCX+). Patterns of exclusive positivity on either the y- or the x-axis (upper left and lower right quadrant) yield CD marker candidates for negative selection strategies. Patterns of shared intracellular and surface expression (upper right quadrant) are suitable for positive selection strategies. Blue outlines illustrate co-expression patterns. (<b>B</b>) Co-localization analysis of the catecholaminergic intracellular marker tyrosine hydroxylase (TH) is shown on BE(2)-M17 cells. The TH-positive subset stained negative for the putative proliferative indicator CD49f but was colocalized with CD90, CD24 and CD200 in this cell line. (<b>C</b>) Co-localization analysis of the glial intracellular marker glial fibrillary acidic protein (GFAP) is shown on SNB-19 cells. In contrast to the other neural cell lines analyzed, GFAP-positive subsets stained positive for CD49f, and also CD90. CD24 was present to a lower degree and the putative neuronal marker CD200 in this context was virtually absent.