Domain 2 of HCV core protein induces lipid accumulation in Huh7 cells.

<p>Huh7 cells were fixed and imaged by CARS and TPF microscopy 24 hours post-transfection with D2-GFP fusion protein. (A) Diagram of the HCV D2-GFP fusion protein. (B)–(E) Simultaneous CARS and two-photon fluorescence image of Huh7 cells expressing D2-GFP. (B) Typical two-photon fluorescence image showing localization of D2-GFP (green); (C) CARS image of lipid droplets (red) in the same field of view; (D) Overlay of the CARS and two-photon fluorescence images; (E) Magnified image from the inset of (D). Representative images are shown of three biological replicates. Scale bar = 10 µm.