Changes in the molecular dynamics simulations due to mutations in the subunits.

<p>The structures are colored according to the difference RMSF values. Colors used are purple, more than 3 standard deviations negative; dark blue, 2 to 3 standard deviations negative; light blue, 1 to 2 standard deviations negative; yellow, +/− one standard deviation; pink, 1 to 2 standard deviations positive; orange, 2 to 3 standard deviations positive; and red, more than 3 standard deviations positive. A. <i>Stu</i>S_Q75A. B. <i>Stu</i>L_Q86A. C. <i>Stu</i>S_W116A. D. <i>Stu</i>L_W128A. The position of the residue mutated to Ala is shown in red, while the non-mutated position is shown in green. The values of the standard deviations were <i>Stu</i>S_Q75A, 0.38; <i>Stu</i>L_Q86A, 0.40; <i>Stu</i>S_W116A, 0.40; and <i>Stu</i>L_W128A, 0.43. Because of the different flexibilities, the Trp-containing loop that was mutated is in different colors. It is shown in red in panel D (arrow).