Immunohistochemical analysis of reporter protein expression in different transgenic mouse lines showed lower expression in the B6N background when compared to FVB.

<p>Cerebellar vibratome slices (cb) of 8-week-old mice were immunolabeled with anti-GFP (A and C) and anti-S100β antibodies (A, C and E), endogenous fluorescence of tdTomato in E. Upper panels depict transgene expression in B6N, lower panels in FVB. The S100β staining indicates all Bergmann glia. Results of comparative analysis in B6N and FVB mice are presented as percentage of transgene expressing Bergmann glia (S100β positive cells) (B, D and F). ***: p<0.001, **: p<0.01. Scale bars indicate 50 µm.