Western blotting confirms expression of PfAtg7 fusion proteins.
- Dawn M. Walker (426407)
- Najmus Mahfooz (34197)
- Katherine A. Kemme (426408)
- Viral C. Patel (426409)
- Maribeth Spangler (426410)
- Mark E. Drew (426411)
Publication date
June 2013
DOIAbstract
<p>(A) PfATG7-HA transgenic parasites confirm protein expression. Western blot of two independent clones detects PfAtg7-HA at ∼150 kDa and ∼65 kDa. Lane 1: PfAtg7-HA clone C1; Lane 2: PfAtg7-HA clone F2; Lane 3: UCH-HA positive control (see Materials and Methods). (B) PfAtg7-RFA transgenic parasites confirm expression of the two PfAtg7 species. Western blot detection of PfATG7-RFA fusion protein incubated in the presence of TMP reveals PfATG7 at ∼200 kDa and ∼100 kDa. RFA tag is 47 kDa.</p
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