IL-6 induced Mcl-1L expression in rat hepatocytes.

<p>(A) Serum levels of IL-6 in PH rats or flavopiridol (2.5 mg/kg) treated for 24 hours before PH group or control group were determined by EIA. Data are provided as mean +/− S.D. *P<0.05. (B) Rat hepatocytes were serum-starved for 24 hours prior treated with recombinant rat IL-6 (0.1, 1 and 10 ng/ml), under serum-starved conditions, and after 4 hours, Mcl-1L protein levels were determined by Western blot analysis. (C) Rat hepatocytes were serum-starved for 24 hours prior treated with recombinant rat IL-6 ( 1 ng/ml), under serum-starved conditions, at the indicated time periods, Mcl-1L protein levels were determined by Western blot analysis. (D) Rat hepatocytes were treated with rat recombinant IL-6 (10 ng/ml) at the indicated time periods. Mcl-1 mRNA expression in rat hepatocytes was determined by q-RT-PCR. *P<0.05.