Dendrogram constructed using DICE for systemic <i>E.</i><i>coli</i>. (tolerance 5%)

<p>(minimum height >0.0%, minimum surface >0.0%)(0.0–100% coefficient) for XbaI PFGE. A dendrogram showing the strain diversity amongst systemic E. <i>coli</i> harbouring APEC VAGs constructed using BioNumerics software by unweighted pair group method with Arithmetic mean. The dendrogram also shows; phylogenetic group (P) (green = D; red = A; yellow = B2; blue = B1), isolate (I), organ and age of bird at isolation (H = heart: K = kidney: Li = liver: Lu = lung; S = spleen), MLST sequence type (ST) and VAG profiles. The dendrogram shows the clustering of ST 117 and 2999 isolates (excluding 601) which by PFGE analysis are ∼60% different from other isolates. Several ST 3004 were identified and these potentially show the acquisition of 2 Iron acquisition genes (<i>irp2</i> and <i>iucD</i>) while other ST 3004 isolates have no VAGs (isolates 579 and 583).