ACT catalytic domain translocation and cAMP production in membranes and cytosol in the presence of calpain inhibitors.

<p>J774A.1 cells were incubated for 10 min with 35 nM ACT in the presence or absence of calpain inhibitors (100 µM calpeptin and 78 nM SJA6017), translocation efficiency (A) and cAMP production in membrane and cytosolic fractions were determined as described in <i>Materials and Methods</i> (B). ***p<0.001 with respect to ACT in the absence of inhibitors.