Chromatin of permeabilised cells compacts with the addition of RNase A.

<p>(A) FLIM images show the decrease in EGFP fluorescence lifetime in permeabilised Hela^H2B-2FP cells that have been treated with RNase A. The increase in compaction is also seen in the intensity images, which show fluorescence from H2b-GFP. (Scale bar –10 µm) (B) The chart shows the average decrease in EGFP fluorescence lifetime normalised to the values of control cells. Error bars indicate standard deviation for values calculated for 13 cells from 3 independent experiments. (* p-value = 9.89×10⁻⁷).