Shows the three day protocols for each fear-conditioning paradigm.

<p>In Experiment 1 baseline sleep (24 hours) data were collected prior to fear conditioning. Subsequently, animals were exposed to either CS alone (tone or 3% CO₂) or CS+US exposures for five repeated series across five time points. CS alone exposures occurred at 9 am and 3 pm, while conditioning trials occurred at 10 am, 12 pm, and 2 pm. An arterial basal blood sample was taken before the first CS exposure and immediately following each of the five series of exposures. The right side shows the three day protocol for Experiment 2. Baseline sleep (24 hours) data were collected prior to fear conditioning. On the subsequent day animals were exposed to paired trials at 12 pm, 2 pm, and 4 pm. One hour following fear conditioning (5 pm), animals began 24 hours of re-exposure to the CS+ (mtHC) or CS− (air) for 60 sec whenever three minutes of consolidated sleep occurred.