Quantitation of length and total region areas of the DAPI-stained brain sections of P1 mice showed significant differences between WT and Ran−/− mice.

<p>Brain sections from level 1 (L1) to level 7 (L7) corresponding to the sections in the Electronic Prenatal Mouse Brain Atlas (EPMBA) were analyzed. The most affected brain area was the volume of the lateral ventricle which was significantly increased in Ran−/− brains at L2 (46-fold), L3 (10-fold), L4 (19-fold) and L5 (17-fold). The cortical plate showed significant decrease in the length at L2 (31%), L3 (47%), L4 (38%) and L5 (46%). The length of total cortex (marginal zone+cortical plate+intermediate zone) was also significantly reduced in Ran−/− brains at L2 (29%), L3 (46%), L4 (36%) and L5 (44%). Marginal zone was decreased only at L1 (39%) and L3 (34%) and the hippocampus only at L4 (49%), but intermediate zone was not affected in Ran−/− brains. One-way ANOVA followed by post-hoc Bonferroni multiple comparisons test revealed significant differences. In each group n = 6, ±SEM. *, p<0.05, **, p<0.01, ***, p<0.001in Ran−/− brains compared to littermate WT controls.