Figure 2

<p><b>A. Caspase 3/7 activity in BW720c-treated uninfected BL20 and infected TBL20.</b> Caspase 3/7 activity was assayed on samples of 10⁴ cells taken from triplicate cultures for each cellular condition; BL 20 and TBL20, untreated and treated with BW720c for 24 and 48 h. Error bars represent the standard deviation from the mean. <b>B. Heat map illustration of the relative expression levels for parasite house-keeping genes before and after exposure to BW720c for 24 and 48 </b><b>h</b> 106 parasite house-keeping genes were randomly selected based on their annotation. The majority of parasite genes show considerably decreased levels by 24 h. Expression level range is 3.9 (blue, low) to 14.9 (red, high). Values are log₂. <b>C. Western analysis demonstrated that expression of parasite proteins Tasp and TA10720 decreased during exposure to BW720c</b> Total protein cell extracts were prepared from untreated BL20 and TBL20 (−) and in TBL20 cells at 24 and 72 (+) hours after addition of BW72c. Equal protein loadings were verified by Ponceau S staining. Parasite proteins, Tasp (schizont surface) and TA10720 (HSP90-related, schizont organelle) are shown relative to constitutive bovine actin. Antibodies are as described in methods section.