DYNLT1 interacts with FG repeat regions of NUP153 and NUP62.

<p><i>A)</i> Full length NUP153, NUP153 deletion mutants, NUP62 and NUP62 deletion mutants. Vertical lines represent FG repeats. <i>B)</i> GST or GST-DYNLT1 immobilized on beads were incubated with <i>in vitro</i> translated NUP153, NUP62, or their deletion mutants. Bound and unbound fractions were resolved by SDS-PAGE and subjected to autoradiography. <i>C)</i> Co-immunoprecipitation of NUP153, NUP62 and NUP98 with DYNLT1 in K562 cells. Whole cell lysates were subjected to immunoprecipitation with either rabbit IgG or anti-DYNLT1 antibody. Both input and immunoprecipitates (IP) were probed for NUP153 or NUP62 using the MAb414 antibody or NUP98 using anti-NUP98 antibody. DYNLT1 was detected with anti-DYNLT1 antibody.