Mapping the strength of association of each tyrosine phosphomotif relevant to RTK pathways with GRB2 from normal breast cells and tumor cells.

<p>Comparative map of active pY motifs on various RTK signaling proteins in breast normal and cancer cells: MCF10A, MCF7, T47D and MDA-MB231. Each bar graph shows relative strengths of each of the tyrosine phospho-motif present in selected RTK pathway proteins interacting with GRB2 as measured by the signal intensity of GRB2 binding to Phosphopeptides (PPEPs) on microarray. The Y axis indicates all the phospho-motifs (source: Phosphosite) on the protein from all four cell lines compared with a control peptide (e.g.EGFR pY988 Vs EGFR Y988A). The X axis indicate intensity of GRB2 binding to each phosphopeptide probes (pY and A peptides). Each value is an average of three independent observations.