Figure 3

<div><p><b>Inverse correlation between EZH2</b> and <b>DLC1 expressions in human cancers.</b></p> <p>(<b>A</b>) Twenty five-paired HCC samples were examined for EZH2 and DLC1 mRNA expression by qPCR. EZH2 was significantly up-regulated in HCC tumorous (T) tissues than non-tumorous (NT) tissues (left panel). In the same sample cohort, DLC1 was significantly down-regulated in HCC as compared to NT tissues (right panel). <i>P</i> values from Wilcoxon matched pair test. (<b>B</b>) An inverse correlation was observed between EZH2 and DLC1 expression in a subset of HCC samples without DLC1 promoter methylation. Expression level of EZH2 and DLC1 in paired-HCC samples was represented by ΔΔCt (T_{(HPRT Ct –Gene of interest Ct)}-NT_{(HPRT Ct – Gene of interest Ct)}). Linear regression analysis was performed using GraphPad Prism5 (La Jolla, CA, USA). (<b>C</b>) EZH2 upregulation (left panel) and concomitant DLC1 downregulation (right panel) was consistently observed in the tumorous tissues of different cancers, including lung [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068226#B32" target="_blank">32</a>], breast [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068226#B33" target="_blank">33</a>], prostate [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068226#B34" target="_blank">34</a>] and ovarian [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068226#B35" target="_blank">35</a>] cancers. Expression data and <i>P</i> values of DLC1 and EZH2 in multiple cancer types was obtained from Oncomine microarray database. Floating bars were shown to illustrate the minimum, median and maximum normalized expression units in non-tumorous (NT) and tumorous (T) tissues.</p>