Quinacrine staning of acidic compartments in <i>M. oryzae</i>.

<p>(<b>A</b>) Observation of acidic compartments during appressorium development of WT strain. WT conidia were allowed to germinate on hydrophobic surfaces and stained by quinacrine at the indicated time points. Bars = 5 μm. (<b>B</b>) Disruption of <i>MoVMA11</i> disturbed vacuolar acidification. Hyphae of WT, <i>ΔMovma11</i>, and the complemented <i>Movma11c</i> strains were cultured on glass slides for 24 h, and then soaked in liquid CM supplemented with 200 μM quinacrine for 15 min. Compared with WT and <i>Movma11c</i> strains, vacuoles of the <i>ΔMovma11</i> mutant were less quinacrine-stained. Bars = 10 μm.