Lineage-specific modulation of miR-125b and miR-93 activity during NSC differentiation.

<p>(<b>A</b>) Representative confocal images of bdLV.CTRL-transduced NSC cultures (mCherry⁺; red, anti-mCherry antibody) showing bright GFP expression (green; direct fluorescence) in neurons (βtubIII, blue) and astroglial cells (nestin, blue). (<b>B,C</b>) Quantification and representative images of miR-125b activity in glial and neuronal subpopulations in bdLV.miRT125b-transduced NSC cultures. (<b>D,E</b>) Quantification and representative images of miR-93 activity in the glial and neuronal subpopulations in bdLV.miRT93-transduced NSC cultures. Data are the mean ±SEM; n = 2 experiments, 2–5 coverlips/antigen/experiment. Prec, <i>stem/precursors</i>; Prog, <i>progenitors</i>; Diff, <i>differentiated cells</i>; d, days in vitro. Arrowheads indicate GFP⁺marker⁺ (miR^−/low) cells; arrows indicate GFP⁻marker⁺ (miR^+/high) cells. Scale bars: 50 µm. Data were analyzed by one-way analysis of variance followed by Bonferroni’s posttest. *, ∧, § p<0.01 versus bdLV.CTRL-transduced GFAP⁺ cells, nestin⁺ and βtubIII⁺ cells, respectively.