DCV levels of <b><i>Wolbachia</i></b><b>-infected flies fed cholesterol-enriched food.</b>

<p>Mean normalised DCV:<i>CycK</i> expression ratios (median ± interquartile range) for <i>w</i>MelPop- (<b>A</b>) and <i>w</i>MelCS-infected flies (<b>B</b>), five days-post DCV infection. Total DCV copies were quantified using qPCR and normalised against <i>CycK</i> expression levels. Data were compared statistically using Mann-Whitney U-tests. Both strains had higher DCV levels after rearing on the Intermediate (Int) and High (High) cholesterol-enriched diets than for Standard diet (Ctrl), this suggests that it is likely that rearing on high cholesterol diets increases the rate of viral accumulation.