Enhanced astrocytosis in IGF-1Tg mice after brain injury.

<p><b>A)</b> Immunoreactivity for GFAP was similar in the hippocampus of WT and IGF-1Tg sham mice. Controlled cortical impact (CCI) brain injury induced astrocytosis in the ipsilateral hippocampus which was more profound in IGF-1Tg mice than in WT mice (shown for 72 h, moderate severity). Boxed areas in the molecular layer of the DG and the apex of the dentate hilus (DH) are magnified in the two lower panels to illustrate hypertrophied astrocytes (arrows) and elongated astroglial processes (arrowheads). <b>B)</b> Representative confocal images from the DH of an IGF-1Tg mouse showing GFAP-stained processes arising from SOX-2 positive cells (arrows). DH: dentate hilus, GL: granule layer. Scale bars = 200 µm for low magnification images and 50 µm (A) or 100 µm (B) for high magnification images.