Contribution of <i>c5032</i>-<i>5039</i> to growth on α-KG and induction of <i>c5032</i>-<i>lacZ</i> or <i>c5038</i>-<i>lacZ</i> expression by α-KG.

<p>(A) <i>In vitro</i> growth of island gene mutants in M9 medium containing α-KG as the sole carbon and energy source. Optical density of the CFT073 WT and mutants during growth in M9 medium containing α-KG as the sole carbon source under anaerobic conditions was determined. Growth bars represent the average measurement at each time point from triplicate experiments. (B) Effects of different substrates on <i>c5032</i>-<i>lacZ</i> or <i>c5038</i>-<i>lacZ</i> expression. Gluc, glucose; Glyc, glycerol; TMAO, Trimethylamine <i>N</i>-oxide. (C) The induction of <i>c5038</i>-<i>lacZ</i> expression by α-KG is dose-dependent. Bacteria were grown in M9 medium containing 0.25% glycerol as the sole carbon source plus different amounts of α-KG as stimulus under anaerobic conditions.