DNA dose-dependent effect of insulin minicircle treatment on hyperglycemia of young diabetic rats.

<p>Five groups of age matched rats (5–7 rats/group) weighing ∼50gm were treated intraperitoneally with 100mg/kg STZ to render them diabetic. Two days after STZ treatment, rats were intravenously injected with indicated dose of TA1m at high pressure. In this experiment, four TA1m doses, 0.25 µg/gm body weight, 0.50 µg/gm body weight, 0.75 µg/gm body weight, and 1.0 µg/gm body weight, were used. A group of untreated diabetic rats and a group of normal rats were included as controls. Blood glucose levels and body weights of each rat were recorded daily; the measurements were alternated between rats fasted and fed <i>ad libitum</i>. These experiments have been repeated at the minimum 3 times. A: All blood glucose level measurements are made on rats fed <i>ad libitum</i>. Orange filled triangle: 0.25 µg/gm TA1m treated STZ-rats; blue filled diamond: 0.50 µg/gm TA1m treated STZ-rats; open black square: 0.75 µg/gm TA1m treated STZ-rats; open green triangle: 1.00 µg/gm TA1m treated STZ-rats; open red diamond: untreated STZ-rats; filled black circle: normal control rats. B: Alternate day measurements of blood glucose levels after a 4 h fast from experimental animals are shown, as indicated.