SAMHD1 inhibits HSV replication.

<p>(A) Western blot analysis of lysates obtained from PMA-stimulated THP-1 shControl and shSAMHD1 cells, infected with varying MOIs of HSV-1 KOS, was performed to determine the levels of SAMHD1, the HSV-1 viral proteins UL-27 and ICP4 (denoted by asterisks), and a tubulin loading control. (B) Real-time PCR was carried out to determine HSV-1 DNA levels after HSV-1 KOS infection in PMA-stimulated shControl (solid line) and shSAMHD1 (dashed line) THP-1 cells at an MOIs of 0.01 over 72 h. (C) PFUs were measured for THP-1 cells infected at an MOI of 0.01. (D) Real-time PCR measured HSV-1 DNA levels for THP-1 cells infected at an MOI of 0.1. (E) PFUs were measured for THP-1 cells infected at an MOI of 0.1. (F) HSV-1 DNA levels were determined after HSV-1 infection of PMA-stimulated shControl and shSAMHD1 THP-1 cells in the presence or absence of 20 µM ganciclovir. The results are representative of two independent experiments performed in duplicate.