The Wnt pathway is involved in BDU-PLM connection.

<p>(A) Quantification of BDU-PLM connection defects in various mutants in the Wnt pathway. (B) The defective BDU-PLM contact phenotype in <i>cam-1</i> and <i>dsh-1</i> mutants. Arrows indicate the BDU and PLM processes. Arrowheads point to the contact region (left panel) or neurite tips (middle and right panels). (C) While <i>lin-44</i> and <i>lin-17</i> affect the polarity of PLM, resulting in shorter PLM neurites, the PLM neurite length is not reduced in <i>dsh-1</i> and <i>cam-1</i> mutants. (D) Ectopic CWN-1 expression (P<i>cwn-2</i>::CWN-1) or over-expression of DSH-1 (OE) in BDU (P<i>unc-53</i>::DSH-1) or PLM (P<i>mec-7</i>::DSH-1) or both BDU and PLM (co-injected with both P<i>unc-53</i>::DSH-1 and P<i>mec-7</i>::DSH-1) causes BDU-PLM contact defects. Error bars in (A, C and D) represent the SEM. *** p<0.001; ** p<0.01; NS, not significant. (E) In wild type, the BDU posterior process contacts the PLM anterior process. The arrowhead indicates the BDU-PLM junction. When CWN-1 is ectopically expressed using P<i>cwn-2</i>, BDU and PLM fail to contact. Arrowheads indicate the tips of the BDU and PLM neurites. Scale bars represent 10 µm.