<i>aha-1</i> directly regulates <i>cam-1</i> gene expression.

<p>(A) Quantification of BDU-PLM contact defects in <i>aha-1(xd4)</i>, <i>ahr-1(ju145)</i>, and <i>cam-1(gm122)</i> single and double mutants. (B) P<i>cam-1b</i>::GFP is expressed in BDU and PLM cells, as indicated by arrows. (C) Fluorescent images and the GFP fluorescence intensity of P<i>cam-1b</i>::GFP in the BDU cell body in wild type and <i>aha-1(xd4)</i>. The GFP fluorescence intensity in BDU cell body is measured using ImageJ and the GFP signal in wild type is normalized to 1. Wild type: n = 35; <i>aha-1(xd4)</i>: n = 54. (D–E) <i>cam-1</i> expression level is decreased in <i>aha-1</i> and <i>ahr-1</i> mutants. The <i>cam-1</i> mRNA level was measured by quantitative RT-PCR and was normalized to 1 in wild type. (F) The association of AHA-1 with the <i>cam-1</i> enhancer region is stronger than with the <i>cam-1</i> coding region (mock). (G) Expression of GFP driven by P<i>cam-1bΔ</i> (<i>cam-1</i> promoter lacking the enhancer region) is not affected by mutation of <i>aha-1</i>. The GFP fluorescence intensity in BDU cell body is measured using ImageJ and the GFP signal in wild type is normalized to 1. (H) Suppression of BDU-PLM connection defects in <i>aha-1</i> and <i>ahr-1</i> mutants by over-expression of <i>cam-1</i> (OE). Scale bars in (B–C) represent 10 µm. Error bars in (A), (D–F), and (H) represent the SEM. *** p<0.001; ** p<0.01; * p<0.05; NS, not significant.