Expression of <i>Elovl4 and CerS3</i> mRNAs in the SS and SG.

<p>Skin samples isolated from E18.5 mouse embryos were fixed with 4% paraformaldehyde, hybridized with a digoxygenin-labeled RNA probe for <i>Elovl4</i>, <i>CerS3</i>, <i>keratin 14</i>, or <i>involucrin</i>, and stained with alkaline phosphatase-conjugated anti-digoxygenin antibody (F_ab fragment) and nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate solution. Frozen sections (25 µm) were subjected to microscopic observation under a DM5000B light microscope and photographed. Bar, 20 µm. De, dermis.